Download JNTU (NR) M.TECH 2-Semester MICROBIAL BIOREACTORS ENGINEERING Exam Question Papers | Indian Shout

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Download JNTU (NR) M.TECH 2-Semester MICROBIAL BIOREACTORS ENGINEERING Exam Question Papers

Code No. B0301
JAWAHARLAL NEHRU TECHNOLOGY UNIVERSITY, HYDERABAD
M .Tech. II Semester Supplementary Examinations, March – 2009
MICROBIAL BIOREACTORS ENGINEERING
(Bio Technology)
Time: 3 hours Max. Marks.60
Answer any Five questions
All questions carry equal marks


1. Write about the Ideal plug flow tubular reactor. Add a note on the relationship among substrate conversion (δ) and reactor design parameters for enzyme catalysed reactions in a PFTR.
2. Discuss about any two different methods for determination of oxygen transfer coefficient (KLa) in detail.
3. Explain different dimensionless groups for fluid-wall heat transfer.
4. Discuss various important factors to be considered in scale-up of microbial bioreactors. What are the difficulties associated with scale-up of a bioreactor.
5. Write about various measuring devices for monitoring and control of the physical environment of the bioreactor.
6. A fermentation is considered for scale up from 101 to 10,000 L vessel. The small fermenter has an aspect ratio of 3. The impeller diameter is 30% of the tank diameter. Agitation speed is 500 rpm and 3 Rushton impellors are used. Determine the dimensions of the large fermenter and agitation speed for.
a) Constant P/v
b) Constant impeller tip speed
c) Constant Reynolds number.
7. Design a plant cell bioreactor. What are its applications?
8. Discuss about the difficulties associated with the designing of a bioreactor for production of antibiotics using fungal cultures.

Code No: B0301
JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD
M.Tech II Semester Regular Examinations, September 2009
MICROBIAL BIOREACTORS ENGINEERING
( Biotechnology )
Time: 3hours Max.Marks:60
Answer any FIVE questions
All questions carry equal marks

– – –
1.a] Explain in detail about CSTR cell reactor with recycle and wall growth with a neat sketch.
b] For the above reactor derive an equation to give the biomass production rate per unit reactor volume and overall (or) external dilution rate.
2.a] What are the reasons for non idealisms , Explain. [4]
b] Write short note on:
a. Exit age distribution [4]
b. F-curve. [4]
3. Write short note on measurement of oxygen transfer coefficient by.
a. Sulfite oxidation method.
b. Dynamic method.
4. Derive the equations to give the power requirements for un gassed Newtonian fluids in laminar zone and turbulent zone and draw the graph between Reynolds Number and Power Number for an un gassed system?
5. Write short note on:
a. Nusselt number
b. Prandtl Number
c. Froude Number
d. Reynolds Number.
6.a] Explain in detail about the plant cells in culture compared to microses.
b] Explain in detail about Bioreactors for suspension cultures.
7. What are the difficulties and some considerations we have to follow in aeration, agitation and heat transfer in the scale up of the bioreactors?
8. A particular fermentation is to be carried out in a chemo stat. Before carrying out the actual fermentation it was decided to evaluate the flow characteristics of the chemo stat by introducing a tracer in the from of pulse input. The time versus concentration data are presented in the table below. Find the average residence time and variance.

TIME(MIN) 0 10 20 30 40 50 60 70
CONCENTRATION OF TRACER IN THE RESPONSE (g/l) 0 2 6 7 4 3 1 0

Code No: 7A201/MT
M.Tech. – II Semester Regular Examinations, September, 2008
MICROBIAL BIOREACTORS ENGINEERING
(Biotechnology)
Time: 3hours Max. Marks:60
Answer any FIVE questions
All questions carry equal marks

– – –
1. Discuss the CSTR designs for enzyme catalysed reactions. Add a note on CSTR cell reactors with recycle.
2.a) Discuss the ‘Dynamic Method’ for measuring the mass transfer coefficient (KLa).
b) A fermenter containing bacterial culture used for the production of an organic acid. Air flow is shut off for a few minutes and the dissolved oxygen level drops. The air supply is then reconnected. When steady state is established, the dissolved oxygen tension is 82% air saturation and the following results are observed.

TIME(sec) 10 25
Oxygen tension

(% air saturation)

60 73

With the given data, estimate the KLa using dynamic method.
3. What is the importance of heat transfer in microbial bioreactors? Give some examples of heat – transfer configurations.
4. Discuss various important factors to be considered in scale – up of microbial bioreactors. What are the difficulties associated with scale – up of a bioreactor?
5. A stirred – tank reactor is to be scaled down from 10m3 to 0.1m3. The dimensions of the large tank are Dt = 2m, Di = 0.5m,
N = 100rpm.
a) Determine the dimensions of the small tank (Dt, Di, H) by using geometric similarity.
b) What would be the required rotational speed of the impeller in the small tank if the following criteria were used.
i) Constant tip speed
ii) Constant impeller Renumber.
6. Write about various possible measuring devices and probes for monitoring and control of chemical environment in a bioreactor.
7. Design a bioreactor for the production of secondary metabolites using non – filamentous fungi. Add a note on the product recovery.
8. Explain about various products of animal cell cultures with suitable examples.

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